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src="/skins/257424/images/ico04.png" />更新時間：2020-05-16</span><span><img src="/skins/257424/images/ico05.png" />點擊次數(shù)：5154</span> </div> <div id="vt10j5u7oi72" class="NyNewDetail_Content" id="c_detail_wrap"> <p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">空白試驗值偏高的原因</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">空白試驗測得的結(jié)果偏高，原因有很多，我們從實際例子來分析原因。</span></span></p><p style="margin-left:0.0000pt"> </p><p style="margin-left:0.0000pt"><strong><span style="font-size:9.5pt"><span style="font-family:arial">一、水中石油類空白</span></span></strong></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">【分析原因】</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">1.四氯化碳對空白值的影響</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">大部分市售四氯化碳都存在空白值高的問題，所以四氯化碳用環(huán)保級，紅外型。</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">2.無水硫酸鈉對空白值得影響</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">用純水處理過的無水硫酸鈉直接用于實驗空白值普遍偏高，經(jīng)過四氯化碳沖洗的無水硫酸鈉測定空白值會有所降低。</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">3.硅酸鎂對空白值得影響</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">將硅酸經(jīng)過四氯化碳預(yù)處理過的空白值也會明顯下降</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">4.漏斗對空白值得影響</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">漏斗和濾紙污染、不潔凈都會使空白值偏高，都需要用四氯化碳預(yù)處理</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">5.器皿清潔度對空白值的影響</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">試劑對空白值得影響很大，但在實驗全過程當中所接觸的全部器皿對空白值得影響也不容忽視。</span></span></p><p style="margin-left:0.0000pt"> </p><p style="margin-left:0.0000pt"><strong><span style="font-size:9.5pt"><span style="font-family:arial">二、水質(zhì)中氨氮空白</span></span></strong></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">【分析原因】</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">1.測定使用的蒸餾水被污染，含有影響實驗結(jié)果的雜質(zhì)，比如蒸餾水含氨。</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">2.試管、移液管等儀器沒有清洗*，這些因素對實驗結(jié)果也有較大的影響。</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">3.實驗所用的無氨水質(zhì)量不佳，含氨量比較高，導(dǎo)致實驗得到的空白值偏高，這是主要的原因。</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">4.在配制酒石酸鉀鈉和鈉氏試劑時出現(xiàn)較大偏差，導(dǎo)致實驗所用的試劑純度（酒石酸鉀鈉、鈉氏試劑）不佳，從而導(dǎo)致實驗得到的空白值偏高。</span></span></p><p style="margin-left:0.0000pt"> </p><p style="margin-left:0.0000pt"><strong><span style="font-size:9.5pt"><span style="font-family:arial">三、總氮空白值偏高的原因？</span></span></strong></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">【分析原因】</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">1、試劑的配制</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">堿性過硫酸鉀的配制過程十分重要，掌握不好，會影響消解效果，對測定結(jié)果產(chǎn)生一定的影響。配制該溶液時，可分別稱取過硫酸鉀和氫氧化鈉，兩者分開配制，再混合定容，或者先配制氫氧化鈉溶液，待其溫度降到室溫后再加入過硫酸鉀溶解。若二者在一只燒杯中溶于水，應(yīng)緩慢加水，同時攪拌，防止氫氧化鈉放熱使溶液溫度過高引起局部過硫酸鉀失效。</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">2、玻璃器皿的洗滌</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">所使用的玻璃器皿應(yīng)先用（1+9）鹽酸浸泡后，再用無氨水沖洗數(shù)次才能使用，否則，也會造成空白值偏高或平行性較差的情況。</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">3、比色時的注意事項</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">該項目的測定涉及兩個波長（220nm和275nm），建議在測定完一組樣品的同一波長后，再調(diào)整到另一波長，統(tǒng)一測定，不要測完一個樣品的兩個吸光度后再換另一個樣品，這樣反復(fù)調(diào)整波長會引起一定的測量誤差。</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">4、試劑的選擇</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">堿性過硫酸鉀消解紫外分光光度法測定總氮的過程中，過硫酸鉀是至關(guān)重要的試劑。首先，試劑的純度關(guān)系到空白值的高低、測定結(jié)果的準確度。一般普通分析純過硫酸鉀的總氮含量不超過0.005%，但由于試劑質(zhì)量存在差異，有些廠家、批次的試劑含氮量常常達不到這個要求，致使空白值偏高。因此，有條件的話建議使用優(yōu)級純試劑，盡量降低試劑中的含氮量，從而降低實驗空白值。</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">5、實驗用水及試劑的質(zhì)量檢驗</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">若實驗的空白值不夠理想，則需要對實驗用水及試劑進行檢驗，以選擇出含氮量低的水和試劑，獲得理想的空白值。</span></span></p><p style="margin-left:0.0000pt"><span style="font-size:9.5pt"><span style="font-family:arial">由于檢測儀器和玻璃量器定期檢定，以及采取了全面的質(zhì)量管理措施，因此，若測定的空白實驗值太大，一般是化學藥品純度不夠，配制的試液變質(zhì)或被污染等原因。應(yīng)重新配制試液，返工重測該批樣品，直至空白實驗值合格。</span></span></p> </div> <div id="vt10j5u7oi72" class="NyPro_PrevNext"> <p class="NyProPrev"><img src="/skins/257424/images/jt10.png" />上一個：<a href="/jsshengaohua-Article-2222950/">比較成熟的COD檢測方法有哪些？</a></p> <a href="/article.html" class="NyProAll"><img 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